anova between treatment groups followed by tukes multiple comparison post hoc test Search Results


statistical software version 5.0  (ESUMI Co Ltd)
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excel toukei ver.7  (ESUMI Co Ltd)
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tukey test  (SYSTAT)
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ptau s396  (Meso Scale Diagnostics LLC)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
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statgraphics.net  (statpoint inc)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
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origin 8.5 software  (OriginLab corp)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
Origin 8.5 Software, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tukey-kramer multiple-comparisons test  (ncss llc)
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ncss llc tukey-kramer multiple-comparisons test
Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
Tukey Kramer Multiple Comparisons Test, supplied by ncss llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chi-square test  (CH Instruments)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
Chi Square Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r-commander  (RStudio)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
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tukey test  (RStudio)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
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tukey multiple comparison test  (RStudio)
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Total Tau and <t>pTau</t> <t>S396</t> protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.
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Image Search Results


Total Tau and pTau S396 protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.

Journal: Frontiers in Neuroscience

Article Title: Modeling Alzheimer’s disease related phenotypes in the Ts65Dn mouse: impact of age on Aβ, Tau, pTau, NfL, and behavior

doi: 10.3389/fnins.2023.1202208

Figure Lengend Snippet: Total Tau and pTau S396 protein level in Ts65Dn and 2N mouse brain total homogenate and insoluble fraction at different ages by Meso Scale Discovery and WB. (A) Schematic representation of the fractions used for evaluation. The total homogenate was used for measuring total Tau and pTau levels. P3-SinT was used for the Sarkosyl insoluble Tau fraction. (B) Total Tau measured by Meso Scale Discovery. Age had a significant effect on levels of total Tau ( p < 0.0001). (C) Total Tau as measured by WB. Age had a significant effect ( p = 0.0218) as did genotype ( p = 0.0218). (D) pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages as measured by Meso Scale Discovery. Age ( p < 0.0001) and genotype ( p = 0.0228) were significant factors for pTau S396 levels. (E) WB evaluation of pTau S396 protein level in total brain homogenates of 2N and TS65Dn mice at different ages measured. Age ( p < 0.0001) was a significant factor for pTau S396 levels. The concentrations of pTauS396 obtained by Meso Scale Discovery were normalized on the total protein concentration, while the results obtained by WB were normalized on the total protein concentration and expressed as the corrected area. (F) pTau S396 protein level in Sarkosyl-insoluble fraction of the 13- and 16-month-old cohort measured by WB. ANOVA indicated a significant effect of genotype ( p = 0.0066), age ( p = 0.0024), and interaction of age and genotype ( p = 0.0066). (G) pTau S396 protein level in Sarkosyl-insoluble fraction of the 16-month-old cohort measured by Meso Scale Discovery. Results are expressed as individual values and mean ± SD.

Article Snippet: Post hoc Tukey’s multiple comparisons tests identified significant increases in pTau S396 levels measured by Meso Scale Discovery in Ts65Dn mice at 10 ( p < 0.0001), 13 ( p < 0.0001), and 16 ( p < 0.0001) months of age compared to 7 months of age, and at 16 months of age compared to 10 months of age ( p = 0.0053).

Techniques: Protein Concentration